Purification of a factor specific for the upstream element of the adenovirus‐2 major late promoter.

Autor: Moncollin, V., Miyamoto, N.G., Zheng, X.M., Egly, J.M.
Zdroj: The EMBO Journal; October 1986, Vol. 5 Issue: 10 p2577-2584, 8p
Abstrakt: Stimulation of in vitro transcription by the upstream element (UE) of the adenovirus‐2 major late promoter (Ad2MLP) involves a specific trans‐acting factor present in a HeLa whole‐cell extract. By following its transcriptional stimulatory activity and its DNase I footprint on the Ad2MLP‐UE, we have purified this factor to greater than 10% purity and separated it from RNA polymerase B and the general transcription factors required for transcription from the Ad2MLP.
Databáze: Supplemental Index