| Autor: |
Varsanyi, M., Tölle, H.G., Heilmeyer, M.G., Dawson, R.M., Irvine, R.F. |
| Zdroj: |
The EMBO Journal; September 1983, Vol. 2 Issue: 9 p1543-1548, 6p |
| Abstrakt: |
Approximately 1 mol phosphatidylinositol phosphate is formed per mol isolated Ca2+ transport ATPase when the enzyme is incubated with ATP/Mg2+. The phosphorylation of this enzyme‐associated phosphatidylinositol represents the alkylphosphate formation described earlier. The phosphatidylinositol phosphate has been found in the hydrophobic core of the enzyme. A complex of phosphatidylinositol phosphate with protein can be extracted with acidic chloroform/methanol. The protein behaves like proteolipid during chromatography on Sephadex LH 60 and binds the radioactively labelled phosphatidylinositol phosphate. The phosphorylation of approximately 1 mol phosphatidylinositol per 100,000 g protein correlates with an enhancement of the Ca2+ transport ATPase activity which is due to an approximately 7‐fold enhanced affinity for Ca2+ and an approximately 2‐fold enhanced maximal turnover. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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