| Autor: |
Fu, Meilan, Wang, Xiangwei, Tang, Shubing, Bai, Shimeng, Ding, Longfei, Hu, Yangyang, Cao, Kangli, Yang, Tianhan, Zhao, Chen, Zhang, Xiaoyan, Xu, Jianqing |
| Zdroj: |
STAR Protocols; December 2024, Vol. 5 Issue: 4 |
| Abstrakt: |
Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, we present a protocol for isolating antigen-specific plasma B cells from immunized mice using Beacon, sequencing the encoded B cell receptors (BCRs), and cloning and expressing the resulting antibodies. This protocol can easily be extended to human samples. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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