| Autor: |
Santativongchai, Pitchaya, Klaeui, Caitlin C., Kosonsiriluk, Sunantha, Saqui-Salces, Milena, Reed, Kent M., Wileman, Ben W., Studniski, Marissa M., Boukherroub, Kahina S. |
| Zdroj: |
STAR Protocols; December 2024, Vol. 5 Issue: 4 |
| Abstrakt: |
The study of reproductive function in turkey hens has been difficult due to the lack of a reliable, representative in vitromodel for investigating profound physiological aspects. This article presents a protocol to establish turkey oviductal organoids, including steps for isolating turkey oviduct epithelial cells followed by seeding and maintaining 3D organoid cultures. We also detail procedures for organoid fixation for histological analysis. This organoid model could serve as a valuable in vitrotool for understanding the intricacies of turkey reproductive physiology. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
