| Autor: |
Ierardi, Rosalie A., Burnum, Annabelle L., Camp, Lauren E., Delaney, Lauren E., Gull, Tamara, Havis, Brett M., Johnson, Gayle C., Kim, Dae Young, Kuroki, Kei, Mammone, Renata M., Mitchell, William J., Navarro, Mauricio A., Rivero, Luis A., Shapiro, Karen, Smith, Amanda C., Valerio, Courtney M., Williams, Fred, Zinn, Michael M., Uzal, Francisco A. |
| Zdroj: |
Journal of Veterinary Diagnostic Investigation; 20240101, Issue: Preprints |
| Abstrakt: |
Bacillary hemoglobinuria (BH) is an infectious disease, mostly affecting cattle, caused by Clostridium haemolyticum(C. novyitype D), with acute hepatic necrosis and intravascular hemolysis. Cattle are typically predisposed to BH by liver injury caused by Fasciola hepatica, although cases have been reported in cattle without evidence of this parasite. Here we describe a cluster of 14 BH cases from 7 counties in north-central to central Missouri submitted to a veterinary diagnostic laboratory between December 2020 and April 2023. Postmortem examination in all cases revealed hemoglobinuria and acute hepatic necrosis with large numbers of gram-positive bacilli with terminal-to-subterminal spores. Flukes, fluke ova, and/or fluke pigment consistent with Fascioloides magnawere identified in 12 of 14 cases. Sequences of the nuclear ribosomal internal transcribed spacer 1 (ITS1) from one fluke had 100% identity to F. magna. C. novyiwas detected by fluorescent antibody testing of liver impression smears (11 of 12 cases) and by immunohistochemistry of liver sections (7 of 7 cases). PCR on formalin-fixed, paraffin-embedded tissues amplified the C. haemolyticumbeta toxin gene in each of the 7 cases tested. To our knowledge, a confirmed cluster of BH associated with F. magnahas not been reported previously in cattle. |
| Databáze: |
Supplemental Index |
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