Detection of serum hepatitis B virus using a nested polymerase chain reaction assay

Autor: Lucotte, G., Galzot, P., Lu, C. Y., Bathelier, C., Champenois, T.
Zdroj: Molecular and Cellular Probes; December 1994, Vol. 8 Issue: 6 p437-440, 4p
Abstrakt: We described a nested polymerase chain reaction (PCR) assay to detect serum hepatitis B virus (HBV) DNA in patients. We compared the sensitivity of the PCR assay to that of slot-blot hybridization for detecting HBV DNA in serum and found that analysis by PCR provides a > 104 fold increase over the slot-blot assay. Most of the HBe Ag-positive patients were positive in PCR, and most of those being anti-HBe-positive were with this PCR assay. Copyright 1994, 1999 Academic Press
Databáze: Supplemental Index