PRIMER NOTE Characterization of eight polymorphic microsatellite loci from the Bengalese finch (Lonchura striata var. domestica)

Autor: Yodogawa, Y., Nishiumi, I., Saito, D., Okanoya, K.
Zdroj: Molecular Ecology Notes; June 2003, Vol. 3 Issue: 2 p183-185, 3p
Abstrakt: Eight polymorphic microsatellite loci were isolated and characterized from the Bengalese finch (Lonchura striata var. domestica). In analyses of 25 individuals, the number of alleles ranged from two to four, and observed heterozygosity ranged between 0.05 and 0.73. At four loci, the observed heterozygosity of the Bengalese finches was significantly different from the expected heterozygosity. Primer sets were also tested in Javan munia (Lonchura leucogastroides), and the same eight loci were successfully amplified. In analyses of 20 unrelated individuals, the number of alleles ranged from one to seven, and the observed heterozygosity ranged from 0 to 0.56. In Javan munia, the observed heterozygosity differed significantly from the expected heterozygosity in only one locus.
Databáze: Supplemental Index