| Autor: |
Dobersberger, Markus, Sumesgutner, Delia, Zajc, Charlotte U., Traxlmayr, Michael W. |
| Zdroj: |
STAR Protocols; December 2024, Vol. 5 Issue: 4 |
| Abstrakt: |
Yeast surface display is a versatile protein engineering technology, enabling precise control of the applied selection pressure. We present a yeast-surface-display-based protocol for the enrichment of binders specifically recognizing ligand-bound receptors. We describe steps for magnetic bead selections, random mutagenesis, and flow cytometric sorting, followed by library sequencing and detailed analysis of enriched clones. While this approach is exemplified with rcSso7d-based libraries and epidermal growth factor (EGF)-epidermal growth factor receptor (EGFR) complexes, it can also be adapted to other binder scaffolds and ligand-receptor systems. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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