| Autor: |
Green, Louis, Waugh, Sheela, Binkley, Jonathan P., Hostomska, Zuzana, Hostomsky, Zdenek, Tuerk, Craig |
| Zdroj: |
JMB Online (Journal of Molecular Biology); March 1995, Vol. 247 Issue: 1 p60-68, 9p |
| Abstrakt: |
We had previously usedin vitroRNA selecti0on techniques to describe a consensus RNA pseudoknot that binds and inhibits HIV-1 reverse transcriptase (HIV-RT). In this work we constructed variants of this consensus pseudoknot in order to evaluate the contributions of individual nucleotide identities and secondary structure to affinity for HIV-RT. We have also used chemical modification of ligand RNAs to corroborate the predicted structure of the pseudoknot, to discover which modifiable groups are protected from chemical attack when bound to HIV-RT, and to find which modifications interfere with binding to HIV-RT. A novel interference study is presented which involves selection of ligands from a pool created by mixed reagent oligonucleotide synthesis in order to rapidly determine allowed substitutions of 2′-OCH3groups for the usual 2′-OH group in such RNA ligands. |
| Databáze: |
Supplemental Index |
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