| Autor: |
Kachwala, Mahera J., Hamdard, Farishta, Cicek, Damla, Dagci, Hilal, Smith, Christopher W., Kalla, Nabeel, Yigit, Mehmet V. |
| Zdroj: |
Advanced Healthcare Materials; September 2024, Vol. 13 Issue: 22 |
| Abstrakt: |
Salmonella, the most prevalent food‐borne pathogen, poses significant medical and economic threats. Swift and accurate on‐site identification and serotyping of Salmonellais crucial to curb its spread and contamination. Here, a synthetic biology cascade reaction is presented on a paper substrate using CRISPR‐Cas12a and recombinase polymerase amplification (RPA), enabling the programming of a standard toehold RNA switch for a genome of choice. This approach employs just one toehold RNA switch design to differentiate between two different Salmonellaserotypes, i.e., S. Typhimurium and S. Enteritidis, without the need for reengineering the toehold RNA switch. The sensor exhibits high sensitivity, capable of visually detecting as few as 100 copies of the whole genome from a model Salmonellapathogen on a paper substrate. Furthermore, this robust assay is successfully applied to detect whole genomes in contaminated milk and lettuce samples, demonstrating its potential in real sample analysis. Due to its versatility and practical features, genomes from different organisms can be detected by merely changing a single RNA element in this universal cell‐free cascade reaction. A highly sensitive universal CRISPR‐Cas12a and toehold RNA switch cascade reaction is developed on a paper substrate for the visual detection of Salmonellagenome in consumable food products. |
| Databáze: |
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