| Autor: |
Margoni, Mudaliar Mahesh, Rajivgandhi, Govindan, Gnanasekaran, Chackaravarthi, Chelliah, Chenthis Kanisha, Sathik Basha, S., Ramachandran, Govindan, Maruthupandy, Muthuchamy, Dhanasekar, M., Quero, Franck, Akbari-Fakhrabadi, Ali, Bhaviripudi, Vijayabhaskara Rao, Kadaikunnan, Shine, Almanaa, Taghreed N. |
| Zdroj: |
Journal of Drug Delivery Science and Technology; October 2024, Vol. 100 Issue: 1 |
| Abstrakt: |
Biofilm architecture is an emerging threat worldwide due to enhanced antibiotic resistance against existing antibiotics, thus heightening the need to discover the alternative therapies that can eradicate the biofilm colonization. Current study highlights the eco-friendly, non-toxic nature of Co3O4nanoparticles (NPs) using Millettia pinnata(M. pinnata) leaves extract and their application in anti-oxidants, anti-bacterial, anti-biofilm and cytocompatibilities. The GC-MS and FT-IR spectral data suggested that phytochemical derivatives of M. pinnataleaf extract simultaneously contributed in bio-reduction process and capping agent of Co3O4NPs, which might be reason for anti-oxidant activities. Further, XRD analysis confirms the polycrystalline behaviour of Co3O4NPs having hexagonal crystal system. FESEM, HRTEM, EDAX and elemental mapping revealed the formation of agglomerated granular shape along with nanorods on the surface of Co3O4NPs, which confirms the presence of carbon and oxygen. Additionally, the characteristic band and thermal decomposition of Raman and TGA spectral data confirmed the molecular structure of Co3O4NPs. Subsequently, both the M. pinnataleaves extract and Co3O4NPs were found to be excellent source of antioxidants and produced significant DPPH and hydroxyl scavenging activities. Furthermore, the sub-biofilm inhibitory concentration of biosynthesized Co3O4NPs reduced the biofilm growth in S. aureusand K. pneumoniaeby crystal violet assay at increasing concentration. In addition, the membrane integrity of biofilm damages and architecture destruction was evidently proved due to the biosynthesized Co3O4NPs by confocal laser scanning electron microscope and scanning electron microscope observation. Finally, the cytotoxicity experiment results indicated, biosynthesized Co3O4NPs has no toxicity against human pulmonary alveolar epithelial cells at maximum concentration. Accordingly, findings of this study supported the utility of safety and efficiency of biosynthesized Co3O4NPs against biofilm producing bacteria, and deserve as promising future drug discovery target for various bacterial infections. |
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