| Autor: |
Fujii, Tatsuya, Ishiya, Koji, Saika, Azusa, Morita, Tomotake |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; September 2024, Vol. 88 Issue: 9 p1109-1116, 8p |
| Abstrakt: |
The basidiomycetous yeast Pseudozyma tsukubaensisis known as an industrial mannosylerythritol lipid producer. In this study, the PtURA5marker gene was deleted by homologous recombination. Using the PtURA5-deleted mutant as a host strain, we obtained a derivative disrupted for the PtKU70gene, a putative ortholog of the KU70gene encoding a protein involved in the nonhomologous end-joining pathway of DNA repair. Subsequently, the introduced PtURA5gene was re-deleted by marker recycling. These results demonstrated that the PtURA5gene can be used as a recyclable marker gene. Although the frequency of homologous recombination has been shown to be increased by KU70disruption in other fungi, the PtKU70-disrupted strain of P. tsukubaensisdid not demonstrate an elevated frequency of homologous recombination. Furthermore, the PtKU70-disrupted strain did not show increased susceptibility to bleomycin. These results suggested that the function of this KU70ortholog in P. tsukubaensisis distinct from that in other fungi.Graphical AbstractCharacterization of PtKU70-disrupted strain constructed by a marker recycling system. |
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