| Abstrakt: |
Legumes are widely recognized for their high protein content and bioactive constituents, they have traditionally constituted a substantial component of human dietary patterns, presenting opportunities for enhanced well-being beyond basic nutrition. This study aimed to assess the bioactive molecule content of Vigna mungo beans grown in southern Algeria, specifically L-DOPA, total phenolic and flavonoid compounds, and their antioxidant capacity. Hydro-alcoholic extract of Vigna mungo whole beans (TADW) and dehulled ones (TADD) were subjected to UPLC/MS/MS quantitative and qualitative determination. The extracts' antioxidant properties were evaluated using DPPH free radical scavenging, ABTS cation radical decolorization, Phenanthroline assay, reducing power assay (FRAP), and Silver Nanoparticle Assay (SNP). L-DOPA content was higher in TADW extract (62.28 ± 1.15 μg/g) than in TADD one (45.00 ± 0.95 μg/g) (p<0.05), along with elevated levels of polyphenols and flavonoids. Twenty-three phenolic compounds were identified in the TADW extract, while the TADD extract contained 17 phenolic elements. TADW extract contained 4-methoxybenzoic Acid, 2-methoxybenzoic Acid, Coumaric acid, and kaempferol as prominent constituents. The TADW extract exhibited the highest antioxidant activity in all tests (IC50 value: 22.14±0.37 μg/mL in DPPH assay, IC50 value: 22.04±0.10 μg/mL in ABTS assay, A0.5 value: 36.94±1.21 μg/mL in SNP assay); however, TADD presented the best antioxidant capacity in FRAP assay (A0.5 value: 21.43±1.76 μg/mL ) and Phenanthroline assay (A0.5 value 14.31±4.17 μg/mL). |
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