Design and Synthesis of Mandelic Acid Derivatives for Suppression of Virulence viaT3SS against Citrus Canker

Autor: Zhang, Yu-Qing, Wang, Xin, Shi, Huabin, Siddique, Faisal, Xian, Jiaxin, Song, Aiting, Wang, Boli, Wu, Zhibing, Cui, Zi-Ning
Zdroj: Journal of Agricultural and Food Chemistry; May 2024, Vol. 72 Issue: 17 p9611-9620, 10p
Abstrakt: Citrus canker, a highly contagious bacterial disease caused by Xanthomonas citrisubsp. citri(Xcc), poses a substantial threat to citrus crops, leading to serious reductions in fruit yield and economic losses. Most commonly used bactericides against Xcclead to the rapid development of resistant subpopulations. Therefore, it is imperative to create novel drugs, such as type III secretion system (T3SS) inhibitors, that specifically target bacterial virulence factors rather than bacterial viability. In our study, we designed and synthesized a series of mandelic acid derivatives including 2-mercapto-1,3,4-thiazole. Seven substances were found to reduce the level of transcription of hpa1without affecting bacterial viability. In vivobioassays indicated that compound F9significantly inhibited hypersensitive response and pathogenicity. RT-qPCR assays showed that compound F9visibly suppressed the expression of XccT3SS-related genes as well as citrus canker susceptibility gene CsLOB1. Furthermore, the combination with compound F9and quorum-quenching bacteria HN-8 can also obviously alleviate canker symptoms.
Databáze: Supplemental Index