| Autor: |
Gross, N, Balmas Bourloud, K, Brognara, C.Beretta |
| Zdroj: |
Experimental Cell Research; November 2000, Vol. 260 Issue: 2 p396-403, 8p |
| Abstrakt: |
Highly malignant neuroblastoma tumors with MYCNamplification have been shown to downregulate the expression of the CD44 adhesion receptor. We have previously shown that MYCNamplified neuroblastoma cell lines either lack CD44 expression or express a nonfunctional, nonhyaluronic acid-binding CD44 receptor. By analysis of cells with manipulated expression of either CD44 or MYCN,we demonstrate that transfection of cells with a CD44 full-length cDNA construct produced a functional receptor in single copy MYCNcells and a nonfunctional CD44 receptor in MYCNamplified cells, similar to the CD44 receptor expressed by cells with enforced MYCN.Analysis of the in vivogrowth properties of the transfectants revealed that the restoration of a functional CD44 receptor in nonamplified cells resulted in the suppression of in vivocell growth, therefore linking the MYCN-related lack of hyaluronic acid-binding function of CD44 to the highly tumorigenic properties of a subset of neuroblastoma cells. |
| Databáze: |
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