| Autor: |
Kawamata, Osamu, Yoshida, Haruhiko, Hirota, Katsutaro, Yoshida, Akira, Kawaguchi, Ryuji, Shiratori, Yasushi, Omata, Masao |
| Zdroj: |
Biochemical and Biophysical Research Communications; February 1996, Vol. 219 Issue: 1 p266-272, 7p |
| Abstrakt: |
We have established a highly sensitive semi-nested PCR assay for the detection ofH. pyloriinfection using gastric juice samples, which can be aspirated with disposable nasogastric tubes. The primers targetingH. pyloriurease A gene were designed based on the sequence conservation analysis of sixteenH. pyloristrains isolated from Japanese patients. The efficacy of the PCR assay, designated as the URA-PCR, was confirmed byin vitroandin vivoassessments. Its sensitivity was 97.5% in the gastric juice samples aspirated from forty patients with provenH. pyloriinfection, and was significantly higher than that obtained with previously described PCR assays. |
| Databáze: |
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