Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia(Diptera: Culicidae)

Autor: da Silva Ferreira, Francisco Augusto, Simões, Rejane de Castro, Ferreira-Keppler, Ruth Leila, Alencar, Jeronimo, Scarpassa, Vera Margarete, Tadei, Wanderli Pedro
Zdroj: Journal of Medical Entomology; May 2020, Vol. 57 Issue: 3 p745-754, 10p
Abstrakt: The eggs of three Amazonian species of the genus Mansonia(Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralisDyar & Knab, 1916 were morphologically different from those of Mansonia titillans(Walker, 1848) and Mansonia amazonensis(Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensisand Ma. titillansare more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.
Databáze: Supplemental Index