| Autor: |
Gruwel, Marco L.H., Kuzio, Bozena, Deslauriers, Roxanne, Kupriyanov, Valerie V. |
| Zdroj: |
Cryobiology; December 1998, Vol. 37 Issue: 4 p355-361, 7p |
| Abstrakt: |
The effect of hypothermia on isolated perfused rat hearts was studied with31P NMR. Hearts were continuously perfused with phosphate-free Krebs–Henseleit buffer while the perfusate temperature was adjusted. Perfusate pH was kept at 7.40 ± 0.02 throughout the experiments. Using the chemical shift difference between PCr and Pithe intracellular pH was estimated. At 36, 20, and 10°C a cytosolic alkalinization at a pH of 7.05 ± 0.04, 7.21 ± 0.05, and 7.40 ± 0.03 was observed, respectively. At 10°C two Piresonances were observed with a separation of 0.25 ppm. This resonance corresponded to a Piresonance of a cellular compartment with a local pH of 7.78 ± 0.06, likely mitochondrial. This additional resonance disappeared upon warming of the hearts back to 36°C. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect