| Autor: |
Hirozawa, Melissa Tiemi, Ono, Mario Augusto, de Souza Suguiura, Igor Massahiro, Bordini, Jaqueline Gozzi, Hirooka, Elisa Yoko, Ono, Elisabete Yurie Sataque |
| Zdroj: |
Brazilian Journal of Microbiology; September 2024, Vol. 55 Issue: 3 p2527-2538, 12p |
| Abstrakt: |
Fusariumverticillioidescauses significant decrease in corn yield and quality, and produces fumonisins, which represent a serious risk to human and animal health. Bacillusspecies can be an effective and environmentally friendly alternative for F. verticillioidesbiological control. In this study, some properties of cell-free supernatants (CFSs) of two Bacillusspp. identified as Bacillus subtilis(NT1, NT2) as well as the antifungal effect against F. verticillioides97L were evaluated. B. subtilisNT1 and NT2 were isolated from commercially available fermented whole soybeans (Nattō). Antifungal activity was observed in both CFSs of B. subtilisisolates (50—59 mm) obtained by co-culture suggesting that antifungal compound production depends on interaction between bacteria and fungi. Cell-free supernatants from the two B. subtilisisolates inhibited mycelial growth (77%—94%) and conidial germination (22%—74%) of F. verticillioides97L. In addition, CFSs caused significant morphological changes such as distorted and collapsed hyphae with wrinkled surfaces and the presence of a large amount of extracellular material compared to the control without CFSs. Both B. subtilisisolates (NT1 and NT2) produced extracellular proteases, biosurfactants and polar low molecular weight compounds that probably act synergistically and may contribute to the antifungal activity. Antifungal compounds showed heat and pH stability and resistance to proteolytic enzymes. Furthermore, antifungal compounds showed high polarity, high affinity to water and a molecular weight less than 10 kDa. These results indicated that the two B. subtilis(NT1 and NT2) have potential as biocontrol agents for F. verticillioides. |
| Databáze: |
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