| Autor: |
Medina, Juan Gerardo Sánchez, Camacho, José Luis Cuéllar, Ruiz Garcia, Jaime, Mira, Alex, Martínez Martínez, Rita Elizabeth, Comas-García, Mauricio, Rangel, Arturo Garrocho, Pozos-Guillén, Amaury, Romo, Saray Aranda |
| Zdroj: |
International Microbiology; 20240101, Issue: Preprints p1-10, 10p |
| Abstrakt: |
Background: Probiotic bacteria inhibit aggregation, biofilm formation, and dimorphism of Candidaspp. However, the effects of a new probiotic, Streptococcus dentisani, on the growth of Candida albicansand Candida glabratabiofilms are unknown. Objective: To determine the effect of S. dentisanion the different phases of C. albicansand C. glabratabiofilm development. Methods: Growth quantification and ultrastructural analyses were performed on biofilms of C. albicansATCC 90028, C. glabrataATCC 2001, and clinical isolates of C. albicansfrom oral candidiasis (CA-C1), caries (CA-CR1), and periodontal pocket (CA-P1) treated with cell suspensions of S. dentisaniCECT 7746. Cell viability was determined by quantifying colony-forming units (CFU/mL). The ultrastructural analyses were done with atomic force microscopy. Results: S. dentisaniinduced a significant reduction (p< 0.05) of CFU/mL of immature and mature biofilm in all strains of C. albicansand C. glabrata. Microscopic analysis revealed that S. dentisanireduced C. albicansdensity in mixed biofilm. The fungus-bacteria interaction affected cell membrane integrity in yeast. Conclusion: For the first time, our data elucidate the antifungal effect of S. dentisanion the development of C. albicansand C. glabratabiofilms, supporting its usefulness as a niche-specific probiotic to prevent and treat oral dysbiosis. |
| Databáze: |
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