| Autor: |
Isah, Musa, Wan Abdul Wahab, Wan-Nor-Amilah, Abdullah, Hasmah, Jamil, Shajarahtunnur, Sul’ain, Mohd Dasuki, Uba, Abdullahi Ibrahim, Zengin, Gokhan, Lahiri, Dibyajit, Edinur, Hisham Atan, Ishak, Wan Rosli Wan |
| Zdroj: |
Advances in Traditional Medicine; December 2024, Vol. 24 Issue: 4 p1157-1168, 12p |
| Abstrakt: |
The antimicrobial properties of the Melaleuca cajuputiplant have been documented. However, the underlying antimicrobial mechanisms remain relatively unexplored. Thus, this study aimed to investigate the antibacterial effects of M. cajuputileaf extract against selected bacterial strains and unveil the potential antibacterial mechanisms of the most potent sub-fraction through time-kill assay, cell morphology studies, and molecular docking analysis. The fractions and sub-fractions were obtained from the methanolic extract of M. cajuputileaf by bioassay-guided fractionation. The antibacterial activity was tested against Staphylococcus aureus, Streptococcus agalactiae, Klebsiella pneumoniae,and Escherichia coliusing broth microdilution assay. The most potent sub-fraction, Melaleuca fraction 2d (MF2d), demonstrated remarkable antibacterial activity with MIC values ranging from 0.063 to 0.25 mg/mL and induced significant cellular damage against the tested bacteria. The chemical characterization of the most potent sub-fraction (MF2d) from methanolic extract of M. cajuputileaf identified five (5) compounds with 2-isopropyl-10-methyl phenanthrene (83.09%) as the major component. In-silicomolecular docking analysis revealed that all the docked ligands showed strong binding propensity towards target bacterial proteins, including DNA gyrase (PDB ID: 1ZI0), dihydropteroate synthase (PDB ID: 1AD1), and D-alanyl transferase (PDB ID: 6O93) with the binding energy ranging from − 6.0 to − 8.4 kcal/mol. The overall findings demonstrated the potential of the M. cajuputiplant as a valuable source of novel antibacterial agents. |
| Databáze: |
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