| Abstrakt: |
The object of this study was to investigate antioxidant, anticancer, antimicrobial and wound healing activities of aqueous extract of R. coriariaL. (sumak) leaves. Antioxidant activity was carried out using 1,1- diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis(3 ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays and the extract showed 97.0 ± 0.74% and 95.7 ± 1.40% radical scavenging activities, respectively. For anticancer activity, cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay against MCF7 breast cancer and L929 mouse fibroblast cells, and the extract was able to inhibit proliferation of MCF7 breast cancer cells with IC50of 129.1 ± 9.27 μg/mL. Wound closure assay revealed that aqueous extract at 1 μg/mL was able to close 79% of wound gap after 24 h with high migration rate. Antimicrobial activity depicted high inhibitory effects against C. albicans(17 mm), E. coli(16 mm), P. aeruginosa(21 mm), S. aureus(17 mm), K. pneumoniae(15 mm). Furthermore, LC-MS/MS results revealed that the leaves contain phenolic compounds and mainly rich in gallic acid (2.82 mg/kg) caffeic acid (6.84 mg/kg) and ascorbic acid (45.84 mg/kg) which may be strongly associated with the remarkable biological activities the extract. |
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