| Autor: |
Giri, Madhu S., Jingade, Pavankumar, Huded, Arun Kumar C., Daivasikamani, S., Mishra, Manoj Kumar |
| Zdroj: |
Indian Phytopathology; 20240101, Issue: Preprints p1-12, 12p |
| Abstrakt: |
Paramyrothecium roridumis a fungal plant pathogen that infects the leaves and stems of nursery-grown coffee seedlings as well as the berries and leaves of field-grown plants. The pathogenic fungus has a diverse range of host plants with cross-infection potential. In the current study, 11 strains of P. roridumwere collected from infested coffee and other host plants commonly grown in the coffee agroecosystem. The genetic variability among the different P. roridumstrains were evaluated using SRAP and SCoT molecular markers, as well as ITS sequencing. Fourteen SRAP and thirteen SCoT primers produced 32.5% and 33.0% polymorphic fragments, respectively. The mean polymorphism information content (SRAP, 0.21; SCoT, 0.23) and resolving power (SRAP, 18.66; SCoT, 18.46) disclosed low genetic variability among the strains. The UPGMA-based dendrogram created using SRAP and SCoT markers placed the 11 P. roridumstrains into two major clusters and sub-clusters, which was supported by principal coordinate analysis (PCoA). Regardless of the marker types utilized, some of the strains are consistently grouped together indicating close affinity between them. Amplification of the ITS region using the ITS1 and ITS4 primer pairs resulted in the 550 bp aligned sequence in 11 P. roridumstrains. Nucleotide sequence analysis revealed a highly conserved 5.8S and polymorphic ITS1 and ITS2 region due to insertions and deletions (indels) and single nucleotide polymorphism. This study demonstrated that molecular methods can be very efficient tools for the effective discrimination of P. roridumstrains. |
| Databáze: |
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