| Autor: |
León-Lara, Ximena, Fichtner, Alina S., Willers, Maike, Yang, Tao, Schaper, Katharina, Riemann, Lennart, Schöning, Jennifer, Harms, Anna, Almeida, Vicente, Schimrock, Anja, Janssen, Anika, Ospina-Quintero, Laura, von Kaisenberg, Constantin, Förster, Reinhold, Eberl, Matthias, Richter, Manuela F., Pirr, Sabine, Viemann, Dorothee, Ravens, Sarina |
| Zdroj: |
The Journal of Experimental Medicine; July 2024, Vol. 221 Issue: 7 pe20231987-e20231987, 1p |
| Abstrakt: |
Preterm infants are at high risk of developing neonatal sepsis. γδ T cells are thought to be an important set of effector cells in neonates. Here, γδ T cells were investigated in a longitudinal cohort of preterm neonates using next-generation sequencing, flow cytometry, and functional assays. During the first year of life, the Vγ9Vδ2 T cell subset showed dynamic phenotypic changes and elevated levels of fetal-derived Vγ9Vδ2 T cells were evident in infants with sepsis. Single-cell transcriptomics identified HLA-DRhiCD83+ γδ T cells in neonatal sepsis, which expressed genes related to antigen presentation. In vitro assays showed that CD83 was expressed on activated Vγ9Vδ2 T cells in preterm and term neonates, but not in adults. In contrast, activation of adult Vγ9Vδ2 T cells enhanced CD86 expression, which was presumably the key receptor to induce CD4 T cell proliferation. Together, we provide a map of the maturation of γδ T cells after preterm birth and highlight their phenotypic diversity in infections. |
| Databáze: |
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