| Autor: |
TURCU, EDMOND, ALLOT, RICK, LISI, NICOLA, BATANI, DIMITRI, BORTOLOTTO, FULVIA, MASINI, ALESSANDRA, MILANI, MARZIALE, BALLERINI, MONICA, FERRARO, LORENZO, POZZI, ACHILLE, PREVIDI, FABIO, REBONATO, LORENZO |
| Zdroj: |
Laser and Particle Beams; July 2004, Vol. 22 Issue: 3 p323-333, 11p |
| Abstrakt: |
An ensemble of new techniques has been developed to study cell metabolism. These include: CO2 production monitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cell metabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2 production allowed us to measure cell metabolic response immediately after irradiation in a continuous and noninvasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert–Bouguet–Beer law. Results obtained on Saccharomyces cerevisiae yeast cells in experiments performed at Rutherford Appleton Laboratory are presented. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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