| Autor: |
Shchyolkina, A.K., Lysov, Yu.P., Il'ichova, I.A., Chernyi, A.A., Golova, Yu.B., Chernov, B.K., Gottikh, B.P., Florentiev, V.L. |
| Zdroj: |
FEBS Letters; January 1989, Vol. 244 Issue: 1 p39-42, 4p |
| Abstrakt: |
The concentration and temperature dependences of the UV and CD spectra of the oligonucleotide 3′-d(ApTpApTpApTpApTpApTp)-O(CH 2) 6O-5′-d(pApTpApTpApTpApTpApT) (eicosamer) in aqueous solution at pH 7 in the presence of 0.5 M NACl were studied. At less than 10 −6M, the eicosamer was shown to form in solution a hairpin with parallel orientation of chains (parallel hairpin). From thermal denaturation profiles [ A260( T)] the thermodynamic parameters, ΔH°, ΔS° and Tmfor parallel hairpin formation were calculated to be −90±8 kJ/mol. −300±20 J · mol −1· K −1and 40.5°C, respectively. The CD spectra of the parallel double helix differed from those of B-form DNA and had characteristic features: decreasing magnitude of the positive maximum at 265 nm and a negative peak at 285 nm. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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