rRNA-protein neighbourhood in Escherichia coli70 S ribosomes and 70 S initiation complex Probing by bifunctional Pt(II)-containing reagent

Autor: Chistyakov, P.G., Abdukayumov, M.N., Veniaminova, A.G., Vladimirov, S.N., Graifer, D.M., Kazakov, S.A., Karpova, G.G.
Zdroj: FEBS Letters; January 1988, Vol. 236 Issue: 1 p246-250, 5p
Abstrakt: The cleavable homobifunctional reagent dichloro[ N, N, N′, N′-tetrakis(2-aminoethyl)-1,6-hexamethylenediamminediplatinum(II)] dichloride was used for studying rRNA-protein cross-links in free 35S-labelled 70 S ribosomes and within initiation complex ribosome · AUGU 6· fMet-tRNA Metf. It was shown that the sets of proteins cross-linked to 16 S and 23 S rRNA in free 70 S ribosomes and in 70 S initiation complex do not differ significantly. The authors are the first to demonstrate most of the 23 S rRNA-protein cross-links and some 16 S rRNA-protein cross-links, in particular those with L7/L12 protein.
Databáze: Supplemental Index