Which Cyclaneusma minusmorphotypes are responsible for needle cast of Pinusspp. in Slovakia?

Autor: Ondruskova, Emilia, Kobza, Marek, Janosikova, Zuzana, McDougal, Rebecca, Adamcikova, Katarina
Zdroj: Journal of Plant Diseases and Protection; October 2024, Vol. 131 Issue: 5 p1665-1672, 8p
Abstrakt: Cyclaneusma needle cast (CNC) is a needle disease which caused deterioration of vitality and reduction in the growth of pines. The disease is caused by the ascomycetous fungus Cyclaneusma minus, which has two well-described morphotypes; C. minussimile and C. minusverum. The distribution and host range of C. minussimile and verum was determined from needle samples and isolates collected throughout Slovakia from 2014 to 2020. Samples from 111 localities, 11 pine host species and 245 trees collected in different types of planting were analysed. It was found, that both morphotypes are present, but C. minusverum is predominantly responsible for CNC in urban and forest plantings in Slovakia. C. minusverum was positively detected in more than 88% of collected samples, whereas C. minussimile was only in four samples from three localities. Morphotype-specific primers were sufficiently sensitive even for new pine-host species. The host range of C. minussimile and C. minusverum was enriched worldwide. C. minusverum was observed in nine host species, whereas C. minussimile was identified only in one. Cyclaneusma niveumwas also recorded and its presence was confirmed through DNA sequencing.
Databáze: Supplemental Index