| Autor: |
Moguilevsky, Nicole, Steens, Martine, Thiriart, Clotilde, Prieels, Jean-Paul, Thiry, Lise, Bollen, Alex |
| Zdroj: |
FEBS Letters; January 1992, Vol. 302 Issue: 3 p209-212, 4p |
| Abstrakt: |
Human recombinant myeloperoxidase was evaluated in a cell-free system for its inactivation properties on the replication of human immunodeficiency virus, HTLV-III B. In the presence of a hydrogen peroxide generating system (glucose and glucose oxidase) and sodium thiocyanate, the recombinant enzyme inhibited virus-induced syncytium formation and viral replication without causing any cytopathic effects on SupT1 reporter cells. In addition, U937 monocytoid cells, chronically infected with HIV1, were exposed to recombinant myeloperoxidase (10 U/ml) and monitored during 48 h for the accumulation of intracellular p24 viral antigen. Under these conditions, the recombinant enzyme significantly reduced intracellular viral replication without affecting cell viability. |
| Databáze: |
Supplemental Index |
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