| Autor: |
Lokhova, I.A., Nevinsky, G.A., Gorn, V.V., Veniaminova, A.G., Repkova, M.V., Kavsan, V.M., Rudenko, N.K., Lavrik, O.I. |
| Zdroj: |
FEBS Letters; January 1990, Vol. 274 Issue: 1 p156-158, 3p |
| Abstrakt: |
The difference in optimal conditions for DNA polymerization catalyzed by AMV reverse transcriplase on poly(A) and poly(dA) templates with d(pT) 10and (pU) 10primers has been found. A comparison of the initiating abilities of d(pT) 10and (pU) 10, primers under optimal conditions for various template · primer complexes has been made. The best template · primer complex was poly(A) · d(pT) 10and the worst was poly(A) · (pU) 10. The lengthening of d (pT) nprimers by a mononucleotide unit ( n=2 10) increases their affinity by a factor of about 2 and 3 in the case of poly(dA) and poly(A) templates, respectively. The affinities ofd (pT) 11–25to the enzyme does not change with the primer length. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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