Expression of cloned cDNA for a major surface antigen of Plasmodium falciparummerozoites

Autor: Odink, Karel G., Lockyer, Michael J., Nicholls, Stephen C., Hillman, Yvonne, Freeman, Robert R., Holder, Anthony A.
Zdroj: FEBS Letters; January 1984, Vol. 173 Issue: 1 p108-112, 5p
Abstrakt: A cDNA library of P. falciparumwas constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparummerozoites.
Databáze: Supplemental Index