| Autor: |
Schadewaldt, Peter, Stapper, Norbert J., Staib, Wolfgang |
| Zdroj: |
FEBS Letters; January 1987, Vol. 217 Issue: 1 p45-48, 4p |
| Abstrakt: |
Adrenergic regulation of phosphoinositide breakdown in rat skeletal muscle was investigated in 30-min incubations with 10 mM LiCl. In rat hemidiaphragms, prelabelled with D- myo-[2- 3H]inositol, addition of α-agonists (epinephrine, norepinephrine, phenylephrine) induced a 5-8-fold increase of [ 3H]inositol monophosphate accumulation. This could be prevented by inclusion of α-antagonists (phentolamine, prazosin). β-Agonists and/or β-antagonists had no effect. Similar experiments with isolated flexor digitorum brevis muscle fibers yielded confirmatory results. Functional integrity of β-receptor mediated processes was suggested by the β-agonist-induced increase of glucose 6-phosphate in hemidiaphragms and cAMP in fiber preparations. The results indicate that phosphoinositide breakdown in differentiated rat skeletal muscle is, at least in part, under α-adrenergic control. |
| Databáze: |
Supplemental Index |
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