The ATP-driven primary Na +pump in subcellular vesicles of Vibrio alginolyticus

Autor: Dibrov, P.A., Skulachev, V.P., Sokolov, M.V., Verkhovskaya, M.L.
Zdroj: FEBS Letters; January 1988, Vol. 233 Issue: 2 p355-358, 4p
Abstrakt: Subcellular vesicles of Vibrio alginolyticushydrolyse ATP and accumulate Na +in an ATP-dependent fashion. The Na +uptake is (i) strongly stimulated by Δψ-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin +K +and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na +accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na +-ATPase in the cytoplasmic membrane of V. alginolyticus.
Databáze: Supplemental Index