| Autor: |
Shinozawa, Takao, Terada, Shoichi, Matsusaka, Hiroshi, Yamashita, Shinsuke |
| Zdroj: |
FEBS Letters; January 1987, Vol. 219 Issue: 2 p293-295, 3p |
| Abstrakt: |
For the identification of the cGMP-sensitive ion channel protein of frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by photoaffinity labeling with [ 3H]cGMP. We found three cGMP binding polypeptides (66 kDa, 92 kDa and 100 kDa) in the membrane protein fraction of ROS. cGMP binding to the 66 kDa polypeptide required the addition of 2 mM CaCl 2. We propose that this polypeptide corresponds to the cGMP-activated channel protein reported by Cook et al. [(1987) Proc. Natl. Acad. Sci. USA 84, 585–589]. The 100 kDa and 92 kDa polypeptides are subunits of the cGMP phosphodiesterase. |
| Databáze: |
Supplemental Index |
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