| Autor: |
Kärgel, H.-J., Stahl, J., Gross, B., Knespel, S., Bielka, H., Saarma, M. |
| Zdroj: |
FEBS Letters; January 1987, Vol. 220 Issue: 1 p126-128, 3p |
| Abstrakt: |
Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with 32P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl 2) resulted in specific binding to a limited set of ribosomal proteins consisting of proteins L3, L4, L6, L13/15 and — to a lesser extent — L7 and L19. Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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