The carbohydrate moiety of aminopeptidase N of rabbit intestinal brush-border membrane

Autor: Massey, Dominique, Maroux, Suzanne
Zdroj: FEBS Letters; January 1985, Vol. 181 Issue: 2 p207-210, 4p
Abstrakt: Endoglycosidase F was used to eliminate the N-linked complex glycans from intestinal aminopeptidase N. The glycans which were probably O-linked remaining after the endoglycosidase F treatment exhibited the human blood group A and H determinants expressed in enzymes from A +or A −rabbits, respectively. The molecular mass estimation of the two types of glycans by SDS-polyacrylamide gel electrophoresis and the sugar composition of aminopeptidase from A +and A −rabbits strongly suggested the presence of eight N-linked complex glycans and two O-linked oligosaccharides bearing the human group antigenicity.
Databáze: Supplemental Index