| Autor: |
Kraushaar, Harald, Hager, Silvia, Wastyn, Marnik, Peschek, Günter A. |
| Zdroj: |
FEBS Letters; October 1990, Vol. 273 Issue: 1-2 p227-231, 5p |
| Abstrakt: |
Plasma and thylakoid membranes were separated and purified from cell-free extracts of the cyanobacteria Anacystis nidulans, Synechocystis6714, Anabaena variabilisand Nostocsp. strain Mac. Immunoblots of the membrane proteins using antisera raised against subunits I–IV of the chloroplast b6/f-complex gave evidence for the presence of a homologous complex in both plasma and thylakoid membranes from the four species of cyanobacteria investigated. Both plasma and thylakoid membranes catalyzed the electron transfer from (exogenous) plastoquinol-9 and NADH to horse heart ferricytochrome c. However, while with plasma membranes these reactions were severely inhibited by low concentrations of antimycin A and rotenone, respectively, the inhibitors were without major effect on thylakoid membranes. The results will be discussed in terms of a possible similarity (analogy and/or homology?) of cyanobacterial plasma membranes to the inner mitochondrial membrane. |
| Databáze: |
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