| Autor: |
Warren, Martin J., Stolowich, Neal J., Santander, Patricio J., Roessner, Charles A., Sowa, Blair A., Scott, A.Ian |
| Zdroj: |
FEBS Letters; January 1990, Vol. 261 Issue: 1 p76-80, 5p |
| Abstrakt: |
Uroporphyrinogen III methylase was purified from a recombinant hemB −strain of E. coliharbouring a plasmid containing the cysG gene. N-terminal analysis of this purified protein gave an amino acid sequence corresponding to that predicted from the genetic code. From the u.v./visible spectrum of the reaction catalysed by this SAM dependent methylase it was possible to observe the sequential appearance of the chromophores of a dipyrrocorphin and subsequently of a pyrrocorphin. Confirmation of this transformation was obtained from 13C-NMR studies when it was demonstrated, for the first time directly, that uroporphyrinogen is initially converted into dihydrosirohydrochlorin (precorrin-2) and then, by further methylation, into a novel trimethylpyrrocorphin. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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