| Autor: |
Shinkarev, V.P., Drachev, A.L., Dracheva, S.M. |
| Zdroj: |
FEBS Letters; January 1990, Vol. 261 Issue: 1 p11-13, 3p |
| Abstrakt: |
A method of decomposing of the absorption spectrum of four-heme cytochrome of a Rhodopseudomonas viridisreaction center preparation into spectra of individual components was used to estimate the degree of the reduction of hemes as a function of redox potential in the medium. The method enables an evaluation of the shape of redox-titration curves of each heme. The redox-titration curves derived by this approach are approximated well by a Nernst equation with n= 1 and E m-values of 360 mV, 312 mV, 20 mV and less than −50 mV. For all of the redox species the values of midpoint potential estimates by the above method are in good agreement with those determined earlier using another procedure [Dracheva et al. (1988) Eur. J. Biochem. 171, 253-264]. The accuracy of deconvolution of data is within the experimental errors of the redox potential measurement. |
| Databáze: |
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