| Autor: |
Ashton, David S., Beddell, Christopher R., Cooper, David J., Green, Brian N., Oliver, Ronald W.A., Welham, Kevin J. |
| Zdroj: |
FEBS Letters; January 1991, Vol. 292 Issue: 1 p201-204, 4p |
| Abstrakt: |
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (α-chymotrypsin, subtilisin Carlsberg and subtilisin BPN′) before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O-acyl enzyme intermediates had been formed. |
| Databáze: |
Supplemental Index |
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