| Autor: |
Im, Mie-Jae, Holzhöfer, Andreas, Böttinger, Heiner, Pfeuffer, Thomas, Helmreich, Ernst J.M. |
| Zdroj: |
FEBS Letters; January 1988, Vol. 227 Issue: 2 p225-229, 5p |
| Abstrakt: |
The role of the βγ-subunits in the interaction of G-proteins was examined with β 1-adrenoceptors purified from turkey erythrocytes and pure βγ-subunits prepared from turkey erythrocytes and bovine brain. On a non-denaturing polyacrylamide gel, the mobility of βγ-subunits was increased when incubated with β 1-adrenoceptor and the β 1-adrenergic agonist l-(−)-isoproterenol, whereas on incubation with the antagonist l-alprenolol the mobility was unchanged. Furthermore, the β 1-adrenoceptor was retarded on a Sephadex G-50 column equilibrated with βγ-subunits and agonist. No retardation occurred in the presence of antagonist. These data suggest a direct interaction of activated β 1-adrenoceptors with isolated βγ-subunits of G-proteins. |
| Databáze: |
Supplemental Index |
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