| Autor: |
Avetisyan, A.V., Dibrov, P.A., Skulachev, V.P., Sokolov, M.V. |
| Zdroj: |
FEBS Letters; January 1989, Vol. 254 Issue: 1 p17-21, 5p |
| Abstrakt: |
The protonophorous uncoupler carbonyl cyanide m-chlorophenylhydrazone was found to inhibit the growth rate of E. coliand maintenance of the membrane potential (Δψ) in intact bacterial cells. Both inhibitory effects of the protonophore were strongly decreased by Na +. A favourable action of Na +could be observed only when cells were grown under conditions of low Δ μH, i.e. at alkaline pH (8.6) or in the presence of the photonophore in growth medium. It was abolished by addition of ΔpH-dissipating agents, i.e. the penetrating weak base diethylamine and the Na +/H +antiporter monensin, to the incubation medium. In inside-out subcellular vesicles isolated from cells grown at alkaline pH, NADH oxidation was shown to be coupled to Na +uptake which was markedly stimulated by protonophore and inhibited by cyanide and HQNO. It is concluded that the growth of E. coliat alkaline pH or in the presence of protonophore gives rise to the induction of a primary respiratory Na +pump(s). |
| Databáze: |
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