| Autor: |
Morgan, T.V., Stephens, P.J., Burgess, B.K., Stout, C.D. |
| Zdroj: |
FEBS Letters; January 1984, Vol. 167 Issue: 1 p137-141, 5p |
| Abstrakt: |
As normally isolated, Azotobacter vinelandiiferredoxin I (Fd I) is a {[4Fe-4S],[3Fe-3S]} protein: (7Fe)Fd I. We report that anaerobic reconstitution of Fd I from its apoprotein yields a protein whose spectra are distinct from those of (7Fe)Fd I and typical of bacterial ferredoxins. We identify this new form of Fd I as a {2[Fe-4S]} protein: (8Fe)Fd I. (8Fe)Fd I is unstable in air and decomposes to give a ∼ 10% yield of (7Fe)Fd I. These results increase the probability that (8Fe)Fd I is the form of Fd I occurring in vivo and that (7Fe)Fd I results from oxidative degradation during purification. |
| Databáze: |
Supplemental Index |
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