| Autor: |
Boutorin, A.S., Vassilenko, S.K., Baklanov, M.M., Nechaev, Yu.S. |
| Zdroj: |
FEBS Letters; January 1984, Vol. 165 Issue: 1 p93-96, 4p |
| Abstrakt: |
Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The ‘dissected’ molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two fragments was formed when the fragment combination (1–28) + (29–76) was used. A strong discrimination in the ligation yield between different nick positions in the same helix is shown. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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