| Autor: |
Abrahamsson, Hakan, Berggren, Per-Olof, Rorsman, Patrik |
| Zdroj: |
FEBS Letters; January 1985, Vol. 190 Issue: 1 p21-24, 4p |
| Abstrakt: |
The effects of the hypoglycemic sulfonylureas tolbutamide and glibenclamide on free cytoplasmic Ca 2+, [Ca 2+] i, were compared with that of a depolarizing concentration of K +in dispered and cultured pancreatic β-cells from ob/obmice. [Ca 2+] iwas measured with the fluorescent Ca 2+-indicator quin2. The basal level corresponded to 150 nM and increased to 600 nM after exposure to 30.9 mM K +. The corresponding levels after stimulation with 1 μM glibenclamide and 100 μM tolbutamide were 390 and 270 nM respectively. K +depolarization increased [Ca 2+] imore rapidly than either of the sulfonylureas. It is suggested that the increased [Ca 2+] iobtained after stimulation by sulfonylureas is due to depolarization of the β-cells with subsequent entry of Ca 2+through voltage-dependent channels. |
| Databáze: |
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