| Autor: |
Morgan, T.V., Stephens, P.J., Devlin, F., Burgess, B.K., Stout, C.D. |
| Zdroj: |
FEBS Letters; January 1985, Vol. 183 Issue: 2 p206-210, 5p |
| Abstrakt: |
The destructive oxidation of aerobically isolated 7Fe Azotobacter vinelandiiferredoxin I [(7Fe)FdI] by Fe(CN) 63−is examined using low-temperature magnetic circular dichroism (MCD) and EPR. The results demonstrate that oxidation of the [3Fe-3S] cluster occurs only after essentially complete destruction of the [4Fe-4S] cluster. It is therefore feasible by controlled Fe(CN) 63−oxidation to obtain a partially metallated form of FdI, (3Fe)FdI, containing only a [3Fe-3S) cluster. The MCD and EPR data demonstrate that the [3Fe-3S] cluster in (3Fe)FdI is essentially identical in structure to that in the native protein. |
| Databáze: |
Supplemental Index |
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