| Autor: |
Lew, Roger R., Garrill, Ashley, Covic, Lidija, Heath, I.Brent, Serlin, Bruce S. |
| Zdroj: |
FEBS Letters; January 1992, Vol. 310 Issue: 3 p219-222, 4p |
| Abstrakt: |
Protoplasts of the filamentous alga, Mougeotia, and the filamentous fungal oomycete, Saprolegnia ferax, exhibit two K +ion channels (2–6 pA) using the patch-clamp technique when the seals are less than 1 GΩ (about 100 MΩ). The membrane potential of the protoplasts was near 0 mV as measured intracellularly with double-barreled micropipettes; thus, inward K +flux is due solely to concentration differences. Although conductances are in the range expected for K +channels, the activity at 0 mV is not seen in other organisms under gigaseal conditions. This paper draws attention to the usefulness of this subsidiary patch-clamp technique and the novel characteristics of ion channels in Mougeotiaand Saprolegnia. |
| Databáze: |
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