| Autor: |
Schnitzler, Christine E., Chang, E. Sally, Waletich, Justin, Quiroga-Artigas, Gonzalo, Wong, Wai Yee, Nguyen, Anh-Dao, Barreira, Sofia N., Doonan, Liam B., Gonzalez, Paul, Koren, Sergey, Gahan, James M., Sanders, Steven M., Bradshaw, Brian, DuBuc, Timothy Q., Febrimarsa, de Jong, Danielle, Nawrocki, Eric P., Larson, Alexandra, Klasfeld, Samantha, Gornik, Sebastian G., Moreland, R. Travis, Wolfsberg, Tyra G., Phillippy, Adam M., Mullikin, James C., Simakov, Oleg, Cartwright, Paulyn, Nicotra, Matthew, Frank, Uri, Baxevanis, Andreas D. |
| Zdroj: |
Genome Research; 2024, Vol. 34 Issue: 3 p498-513, 16p |
| Abstrakt: |
Hydractiniais a colonial marine hydroid that shows remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractiniaspecies, Hydractinia symbiolongicarpusand Hydractinia echinata, placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpusand identified cell-type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia’s i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractiniahas a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia’s stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractiniapresented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from nonself. |
| Databáze: |
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