| Autor: |
Arp, Alex P, Williamson, Megan E, Vasquez, Mario, Quintero, Gladys, Vargas Lowman, Aidamalia, Sagel, Agustin, Scott, Maxwell J |
| Zdroj: |
Journal of Economic Entomology; April 2024, Vol. 117 Issue: 2 p650-659, 10p |
| Abstrakt: |
Transgenic insect strains with tetracycline repressible (Tet-Off) female-lethal genes provide significant advantages over traditional sterile insect techniques for insect population control, such as reduced diet and labor costs and more efficient population suppression. Tet-Off systems are suppressed by tetracycline-class antibiotics, most commonly tetracycline (Tc) or doxycycline (Dox), allowing for equal sex ratio colonies of transgenic insects when reared with Tc or Dox and male-only generations in their absence. Dox is a more stable molecule and has increased uptake than Tc, which could be advantageous in some insect mass-rearing systems. Here, we evaluated the suitability of Dox for rearing Tet-Off female-lethal strains of Australian sheep blowfly, Lucilia cuprina(Wiedemann, 1830) (Diptera: Calliphoridae), and New World screwworm, Cochliomyia hominivorax(Coquerel, 1858) (Diptera: Calliphoridae), and the effects of dosage on strain performance. For both species, colonies were able to be maintained with mixed-sex ratios at much lower dosages of Dox than Tc. Biological yields of C. hominivoraxon either antibiotic were not significantly different. Reduction of Dox dosages in C. hominivoraxdiet did not affect biological performance, though rearing with 10 or 25 μg/mL was more productive than 50 μg/mL. Additionally, C. hominivoraxmating performance and longevity were equal on all Dox dosages. Overall, Dox was a suitable antibiotic for mass-rearing Tet-Off female-lethal L. cuprinaand C. hominivoraxand was functional at much lower dosages than Tc. |
| Databáze: |
Supplemental Index |
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