| Autor: |
Farberman, M. M., Hoffmann, J. W., Ryerse, J. S., deMello, D. E. |
| Zdroj: |
Inflammation Research; 20040901, Vol. 53 Issue: 9 p475-483, 9p |
| Abstrakt: |
Objective and design: To demonstrate a diffusible intercellular macrophage activation factor secreted by Type II alveolar epithelial cells (AECs) in transwell co-cultures. Materials: T 7, our Type II conditionally immortalized AEC line; MH-S, an alveolar macrophage cell line; Lipopolysaccharide (LPS) or uv-killed Escherichia coli (UVEC) for antigen presentation. Methods: LPS or UVEC stimulation of T 7 cells in the lower chamber was investigated for ability to activate MH-S cells in the upper chamber, as assayed by nitric oxide production and western blots for inducible nitric oxide synthase-2. Results: Both transwell and UVEC-conditioned medium experiments showed secretion of an MH-S activation factor by T 7 cells. Many common inflammatory cytokines were ruled out as this immunoactivator. Conclusion: Demonstration of a diffusible activation factor produced by Type II AECs supports their potential role as first responders of innate immunity in the lung. |
| Databáze: |
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